Opioid Receptors: Methods and Protocols
serves as a comprehensive guide to both key new techniques and
established methods for the investigation of genetics, structural
biology, transcription, and post-transcriptional events of opioid
receptors. Other methods cover the cellular detection and trafficking of
opioid receptors in vitro and in vivo. Procedures aimed
to investigate signaling pathways modulated by opioid receptors and
model systems to study opioid receptor-mediated functions are also
included. Finally, methods to assay behavioral effects mediated by
opioid receptors are described. Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
introductions, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips on
troubleshooting and avoiding known pitfalls.
Wide-ranging and authoritative, Opioid Receptors: Methods and Protocols will help both experienced and new entrants in this field to carry out their experiments successfully and with new inspiration.
(Chapter 1 ). The recent publication of crystal structures
of all the three opioid receptors has been instrumental to the
development of computational protocols, designed to estimate
thermodynamic and kinetic parameters describing the receptor binding of
small molecule ligands and the formation of supramolecular complexes.Wide-ranging and authoritative, Opioid Receptors: Methods and Protocols will help both experienced and new entrants in this field to carry out their experiments successfully and with new inspiration.
(Chapter 2 ). Furthermore, techniques for the epigenetic and posttranscriptional analysis of opioid receptor genes are presented.
(Chapters 3 and 4 ). Finally, a protocol is dedicated to the use of DNA microarrays and next-generation sequencing methodologies to obtain a transcriptional profi le of genes influenced by activation of opioid receptors.
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